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Jitendra Singh Rathore

Jitendra Singh Rathore
Associate Professor
Qualification
M. Sc. Biotechnology (IIT, Roorkee); Ph. D. Biotechnology (JNU and ICGEB, New Delhi)
Email
jitendra.rathore@nsut.ac.in

 

 

 

Dr. Jitendra Singh Rathore did graduation in Life Sciences followed by Post Graduated Diploma in Biochemical Technology from University of Delhi. Later, he joined Indian Institute of Technology (IIT), Roorkee with studentship scholarship from Department of Biotechnology, New Delhi for M.Sc. program in Biotechnology. After qualifying NET-LS, NET-JRF and GATE, he joined Prof. Rakesh Bhatnagar’s lab at School of Biotechnology, Jawaharlal Nehru University (JNU) and International Centre for Genetic Engineering and Biotechnology (ICGEB) New Delhi an United Nation Institute, for his Ph.D. program. He recipient of international travel grant from Council of Industrial Research (CSIR), New Delhi to attend American Society for Cell Biology (ASCB) conference at Washington DC, USA, in 2007. He is also a predoctoral travel awardee from American Society for Cell Biology (ASCB), USA. He is recipient of prestigious Fulbright postdoctoral fellowship from United States-India Education Foundation (USIEF), USA and did postdoc from Perelman School of Medicine, Department of Microbiology, University of Pennsylvania, Philadelphia, USA as Fulbright Postdoctoral Fellow. He has 20 years of experience in molecular bacteriology and genetics. He is proficient in gene cloning, expression and purification of recombinant proteins using AKTA explorer. He also has expertise in Primer Extension, FACS, Western blotting, Site Directed Mutagenesis, Southern and Northern hybridization. His area of research is to decipher the role of Toxin-Antitoxin (TA) modules in biofilm formation, antibiotic resistance, and persister cell formation using TAomics approach. He is using Xenorhabdus nematophila and Pseudomonas aeruginosa and Acinetobacter baumannii as model system. His research is funded by Science and Engineering Research Board (SERB)-Department of Science and Technology (DST), Govt of India, Council of Scientific and Industrial Research (CSIR) New Delhi, India and Indian Council of Medical Research (ICMR), New Delhi, India. 

Areas of Interest:

  • Bacterial Persistence & Host-Pathogen Interaction (Pulmonary Infection Th17 response).
  • Antimicrobial Resistance.
  • Toxin-Antitoxin Biology in Prokaryotes.
  • Computer Aided Drug Design.
  • Vaccine Design and Development.

Publication in International Journals:

  1. Jhinjharia D, Juneja P, Srivastava G, Lokhande KB, Sharma A, Rathore JS, Kaur S, Sahi S. (2025) In-silico guided identification and in-vitro studies of potential FFAR4 agonists for type 2 diabetes mellitus therapy. Expert Opin Drug Discov. Aug;20(8):1075-1092. doi: 10.1080/17460441.2025.2522896. Epub 2025 Jun 28. PMID: 40580106.
  2. Gupta N, Chauhan K, Singh G, Chaudhary S, Rathore JS (2025). Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics. The Microbe. 100233, https://doi.org/10.1016/j.microb.2025.100233.
  3. Gupta N, Yadav M, Ali W, Singh G, Chaudhary S, Grover S, Chandra S, Rathore JS. (2025) Genomic profiling and molecular dynamics analysis of parDEPa toxin-antitoxin homologs targeting DNA gyrase in Pseudomonas aeruginosa: insights from computational investigations. J Biomol Struct Dyn. Jan 1:1-17. doi: 10.1080/07391102.2024.2446675. Epub ahead of print. PMID: 39743786.   
  4. Gupta N, Yadav M, Singh G, Chaudhary S, Ghosh C, Rathore JS. (2024) Decoding the TAome and computational insights into parDE toxin-antitoxin systems in Pseudomonas aeruginosa. Arch Microbiol. Jul 27;206(8):360. doi: 10.1007/s00203-024-04085-2. PMID: 39066828.


 

  1. Chaudhary S, Ali W, Yadav M, Singh G, Gupta N, Grover S, Ghosh C, Chandra S, Rathore JS. (2024) Computational exploration of the genomic assignments, molecular structure, and dynamics of the ccdABXn2 toxin-antitoxin homolog with its bacterial target, the DNA gyrase, in the entomopathogen Xenorhabdus nematophila. J Biomol Struct Dyn. 2024 Feb 7:1-15. doi: 10.1080/07391102.2024.2311337. Online ahead of print


 

  1. Chaudhary S, Singh G, Gupta N, Ghosh C , Rathore JS. (2023) New face in the row of bioactive compounds and toxin-antitoxin modules: Xenorhabdus nematophila. Journal Asia-Pacific Entomology Volume 26, Issue 4, December 2023, 102148


 

  1. Rana S, Kaushik D, Singh A, Gautam D, Rai J, Rathore JS (2023) Aptamer: A theranostic approach towards breast cancer  Clinical Immunology Communications Volume 3, December 2023,  61-73 


 

  1. Mishra S, Arsh AM and Rathore JS (2022) Trained Innate Immunity and Diseases: Bane with the Boon. Clinical Immunology Communications Volume 2, December 2022, Pages 118-129


 

  1. Chaudhary S, Yadav M, Mathpal S, Chandra S and Rathore JS (2022).Genomic assortment and interactive insights of the chromosomal encoded control of cell death (ccd) toxin-antitoxin (TA) module in Xenorhabdus nematophila Journal of Biomolecular Structure and Dynamics doi.org/10.1080/07391102.2022.2114940


 

  1. Yadav M, Rathore JS (2022) In-silico analysis of genomic distribution and functional association of hipBA toxin-antitoxin (TA) homologs in entomopathogen Xenorhabdus nematophila Journal Asia-Pacific Entomology. Volume 25, Issue 3, September 2022, 101949doi.org/10.1016/j.aspen.2022.101949


 

  1. Yadav M, Rathore JS (2021) Functional and transcriptional analysis of chromosomal encoded hipBAXn2 type II toxin-antitoxin (TA) module from Xenorhabdus nematophila. Microb Pathog 2021 Nov 25;162:105309.doi: 10.1016/j.micpath.2021.105309.


 

  1. Singh G, Yadav M, Chaitali Ghosh and Jitendra Singh Rathore  (2021) Bacterial toxin-antitoxin modules: classification, functions, and association with persistence. Current Research in Microbial Sciences 2021 Jul 7;2:100047. doi: 10.1016/j.crmicr.2021.100047. e Collection 2021 Dec.

  

  1. Rathore JS and Ghosh C (2020) Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), a newly emerged pathogen: an overview. Pathog Dis. 2020 Aug 1;78(6)


 

  1. Nim JS≠, Yadav M≠, Gautam LK, Ghosh C, Sahi S and  Rathore JS (2020) Novel toxin-antitoxin system Xn-mazEF from Xenorhabdus nematophila: identification, characterization and functional exploration. Current Computer Aided Drug Design 2020 Jun 25.doi: 10.2174/1573409916666200625135850


 

  1. Yadav M, Rathore JS (2020) The hipBA(Xn) operon from Xenorhabdus nematophila functions as a bonafide toxin-antitoxin module. Appl Microbiol Biotechnol. 2020 Apr;104(7):3081-3095


 

  1. Yadav M and Rathore JS (2018) TAome analysis of type-II toxin-antitoxin system from Xenorhabdus nematophila. Computational Biol and Chem 2018 Oct; 76:293-301


 

  1. GautamLK, Yadav M, and RathoreJS. (2017) Functional annotation of novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach. J Mol Model 23 (6): 189 


 

  1. Pandey DK, Kumar A, Rathore JS, Singh N, Chaudhary B (2017) Recombinant overexpression of dihydroneopterin aldolase catalyst potentially regulates folate-biofortification.   J Basic Microbiol.  Apr 18. 57(6):517-524


 

  1. Rathore JS and Yan Wang (2016) Protective role of Th17 cells in pulmonary infection. Review. Vaccine 34(13): 1504-1514


 

  1. GautamLK, YennamalliRM, Rathore JS (2016) Implication on the function of novel Xn-relE toxin structure of Xenorhabdus nematophila using Homology modeling. Current Bioinformatics 12(6): 535 - 542


 

  1. Rathore JS and GautamLK. (2014) Expression, purification and functional analysis of novel relE operon from X. nematophila. The Scientific World Journal Volume 2014 (2014)  428159 


 

  1. Rathore JS. (2013) Expression, purification and functional characterization of atypical xenocin, its immunity protein and their domains from Xenorhabdus nematophila. International Journal of Bacteriology Volume 2013 (2013), Article ID 746862 


 

  1. Rathore JS. (2013) Function inferences from a molecular structural model of YoeBXn toxin from Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2): 25-29


 

  1. Rathore JS*, Singh MP, and Gautam P. (2013) Insilico analysis of novel hipAB, ccdBA, and yoeB-yefM Toxin-Antitoxin homologs from the Genome of Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2):11-20 (*Corresponding author) 


 

  1. Singh, J. (2013) Role of D535 and H538 in endogenous toxicity of xenocin from Xenorhabdus nematophila. FEMS Microbiology Letters 338: p 147-154. 


 

  1. Sameer Giri and Singh, J*(2013) New Face in the Row of Human therapeutics: Bacteriocins. Journal of Microbiology Research, 3 (2) p71-78 (*Corresponding author)


 

  1. Singh J. (2012) Structural and Functional Interferences from a Molecular Structural Model of Xenocin Toxin from Xenorhabdus nematophila. American Journal of Bioinformatics Research. 2 (4):p55-60 


 

  1. Singh, J*., Chaudhary, R, K., Gautam, P. (2012) Insilico analysis of novel relB, relE and mazF toxin-antitoxin homolog’s from the genome of Xenorhabdus nematophila. 2(3): p21-32. American Journal of Bioinformatics Research (*Corresponding author) 


 

  1. Singh, J*. and Ghosh C. (2012) Ribosomal Encoded Bacteriocins: Their functional insight and applications. Journal of Microbiology Research 2(2): p19-25 (*Corresponding author) 


 

  1. Singh, J. and Banerjee, N. (2008) Transcriptional analysis and functional characterization of a gene pair encoding iron-regulated xenocin and immunity proteins of Xenorhabdus nematophila. Journal of Bacteriology, 190: p3377-85 


 

  1. Banerjee, J.,* Singh, J*, Mohan Chandra Joshi, ShubhenduGhosh, and Nirupama Banerjee. (2006) The Cytotoxic Fimbrial Structural Subunit of Xenorhabdus nematophila Is a Pore-Forming Toxin. Journal of Bacteriology. 188(22): 7957-7962. (*equal contribution).

 

  1. Singh, J., Joshi, M.C., and Bhatnagar, R. (2004). Cloning and expression of glutamine synthetase gene from mycobacterium in E. coli. Biochemical and Biophysical Research Communication. 317: 634-638

Publication in National Journal

  1. Ghosh C,  Kumari H and Rathore JS, "Prevalence and risk of listeriosis by ready to eat products", International Journal of Scientific Research Letters Vol 1, Issue 1, Dec 2017

 

  1. Sharma P, Ghosh C, Rathore JS. Anthocyanin: Structure, Synthesis and its Beneficial Role in Health. Research & Reviews: Journal of Food Science and Technology. 2016; 5(1): 45–55p.

 

Publication in National Conferences

1. Jitendra Singh Rathore. (2023) Role of genome encoded type II Toxin-Antitoxin (TA) module(s) from Pseudomonas aeruginosa in antibiotic resistance and persister cell formation. Poster presented in 63rd Annual Conference of Association of Microbiologists of India (AMI) 2nd -4th February, Maharishi Dayanand University, Rohtak

 

2. Shobhi Chaudhary, Garima Singh, Nomita Guptaand Jitendra Singh Rathore (2023) Study of Chromosomal Encoded ccdABXn1 Toxin-Antitoxin (TA) Module from Xenorhabdus nematophila: An in-silico approach. Poster presented in 63rd Annual Conference of Association of Microbiologists of India (AMI) 2nd -4th February, Maharishi Dayanand University, Rohtak

  1. Garima Singh, Shobhi Chaudhary, Nomita Gupta and Jitendra Singh Rathore.(2023) Functional and Interactive Insights of the Chromosomal Encoded yefM-yoeBXn Toxin-Antitoxin (TA) Module from Xenorhabdus nematophila. Poster presented in 63rd Annual Conference of Association of Microbiologists of India (AMI) 2nd -4th February, Maharishi Dayanand University, Rohtak
  1. Garima Singh and Jitendra Singh Rathore. (2022) Insilico characterization of yefM -yoeBXn toxin-antitoxin module from Xenorhabdus nematophila. Poster presented in 62nd Annual Conference of Association of Microbiologists of India (AMI) 21st -23rd September, University of Mysore.

 

  1. Shobhi and Jitendra Singh Rathore. (2022) Structural and functional nalyis of ccdABXn2 toxin-antitoxin module from Xenorhabdus nematophila. Poster presented in 62nd Annual Conference of Association of Microbiologists of India (AMI) 21st -23rd September, University of Mysore.

 

  1. Nomita Gupta and Jitendra Singh Rathore. (2022) Identification and in silico characterization of chromosomal parDEPa toxin-antitoxin module from Pseudomonas aeruginosa. Poster presented in 62nd Annual Conference of Association of Microbiologists of India (AMI) 21st -23rd September, University of Mysore.

 

  1. Jogendra Singh Nim, Mohit Yadav and Jitendra Singh Rathore (2019). Identification, characterization and functional inferences of a novel Xn-MazEF toxin-antitoxin system from Xenorhabdus nematophila, at Biogenesis-VI: Synergy between Academia and Industry, IILM College of Engineering and Technology, Greater Noida, India, 12-13 April 2019.

 

  1. Mohit Yadavand Jitendra Singh Rathore. (2017). TAome analysis of type-II toxin-antitoxin system from entomopathogenic bacteria Xenorhabdus nematophila. Abstract accepted for Poster presentation at 58th International Annual Conference of The Association of Microbiologists of India (AMI- 2017) at Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh. 

 

  1. Mohit Yadav and Jitendra Singh Rathore. (2017). In-silico interactomics analysis of the Xn-HipAB TA module: structural and gene ontological inferences from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. (BEST POSTER AWARD) 

 

  1. Anjali Gautam and Jitendra Singh Rathore (2017). Homology modeling based structural assessment and functional derivation of novel YoEB toxin from Xenorhabdus nematophila and its interaction with YefM antitoxin. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. (BEST POSTER AWARD) 

 

  1. Deeksha Duggal, Mohit Yadav, and Jitendra Singh Rathore. (2017).  Identification and characterization of putative Xn-FIC- PHD toxin-antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India.

 

  1. Gaurav Kant, Mohit Yadav, and Jitendra Singh Rathore. (2017). Gene ontology study of novel putative Xn-GNAT- RHH toxin-antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 

 

  1. Kirti Vashisth, Mohit Yadav and Jitendra Singh Rathore. (2017).  In-silico structural and functional analysis of the novel Xn-MosAT module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 

 

  1. Anamika Chaudhary, Mohit Yadav, and Jitendra Singh Rathore. (2017). 3D modeling and functional inferences of a novel Xn-PIN- PHD like toxin-antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 

 

  1. Shubhrankita Pandey, Mohit Yadav, and Jitendra Singh Rathore. (2017). 3D structure and function prediction of a novel Xn-GNAT- RHH Toxin-antitoxin system from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 

 

  1. Rupal Chaudhary, Mohit Yadav, and Jitendra Singh Rathore. (2017). In-silico characterization of putative Xn-RelE- RHH Toxin-Antitoxin Module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propagation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 

 

  1. Mohit Yadav1, Chaitali Ghosh2, Jitendra Singh Rathore(2015) Functional and transcriptional analysis of novel Xn-relB and Xn-relE toxin-antitoxin modules from Xenorhabdus nematophila. School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India, 2Department of Zoology, Gargi College, University of Delhi, New Delhi, India.) Poster presented in 56th Annual Conference of Association of Microbiologists of India (AMI) 7th -10th December, Jawaharlal Nehru University, JNU, New Delhi, India 2015

 

  1. Mohit Yadav1, Jitendra Singh Rathore (2015). Cloning and Evaluation of LacZ  activity of relB/relE toxin-antitoxin (TA) modules of bacterium Xenorhabdus nematophila .School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India) Poster presented in Industrial-Academia Meet in Gautam Buddha University, Greater Noida, Uttar Pradesh, 2015 (Won First Prize)


 

Publication in International Conferences

  1. Garima Singh and Jitendra Singh Rathore. (2023) Structural and Functional annotation of novel gnat-rhh Xn Toxin-Antitoxin Module from Xenorhabdus nematophila. Poster presented in Antimicrobial Resistance and Human Microbiome International Conference. 15 th -18 th November, Translational Health Science and Technology Institute (THSTI), Haryana.


 

  1. Kanchan Chauhan and Jitendra Singh Rathore. (2023) In-silico analysis of parE-parD Toxin- Antitoxin Module from Pseudomonas aeruginosa. Poster presented in Antimicrobial Resistance and Human Microbiome International Conference. 15 th -18 th November, Translational Health Science and Technology Institute (THSTI), Haryana

 

  1. Mohit Yadav and Jitendra Singh Rathore (2019). Functional and transcriptional characterization of novel Identified hipBAXn toxin-antitoxin (TA) module from Xenorhabdus nematophila Abstract accepted for Poster presentation at ASM Microbe 2019 at San Francisco United States of America (USA), 20-24 June 2019. 

 

  1. Jitendra Singh Rathore (2017). Study of Th17 antigens from Streptococcus pneumoniae at International Vaccine Conference at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 27-29 Nov 2017 

 

  1. Mohit Yadavand Jitendra Singh Rathore. (2017). Structural and functional inferences of a novel hipBAXn toxin-antitoxin module from Xenorhabdus nematophila. Abstract accepted in INSCR International Conference (IIC-2017) University of Delhi 26-28 September 2017. 

 

  1. Singh, J. 1,2 and Banerjee, N. 2 (1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India.2 International Centre for Genetic Engineering and Biotechnology, New Delhi, India.) Role of D535 and H538 in endogenous toxicity of xenocin from Xenorhabdus nematophila. Poster presented at 111th General meeting American society of for microbiology (ASM), New Orleans, Louisiana May 21-24, 2011.

 

  1. Banerjee, N.,1 Singh, J., 1 Singh, P., 1 Forst, S., 2 and Park D. 2 (1Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, 2. Department of Biological Sciences, University of Wisconsin, Milwaukee, WI) “Xenocin Export in Xenorhabdus nematophila”. Abstract accepted for 49th annual meeting of American Society for Cell Biology (ASCB) at San Diego, CA December 5-9, 2009.

 

  1. Singh, J., Banerjee, N., and Bhatnagar R. "Secretion studies of xenocin operon from Xenorhabdus nematophila” Abstract accepted for 109th General meeting of American society for Microbiology (ASM) at Philadelphia, Pennsylvania, USA May 2009.

 

  1. Singh, J., Banerjee, N., and Bhatnagar R." Transcriptional analysis and functional characterization of a gene pair encoding iron-regulated xenocin and immunity proteins of Xenorhabdus nematophila” poster presented at Annual Conference of American Society for Cell Biology (ASCB) at Washington DC, USA December 2007.

 

Book/Book Chapters:

1. Jitendra Singh Rathore and Chaitali Ghosh (2018). "Pathogen-Associated Molecular Patterns and Their Perception in Plants", chapter published in the book titled "Molecular Aspects of Plant-Pathogen Interaction", published by Springer Nature, ISBN 978-981-10-7370-0    ISBN 978-981-10-7371-7 (eBook) 

 

  1. Chaitali Ghosh and Jitendra Singh Rathore (2020). "Molecular Biology of Thermophilic and Psychrophilic Archaea" in Microbiomes of Extreme Environments Biodiversity and Biotechnological Applications published by CRC press Taylor and Francis ISBN 978-036-73-4274-6 November 14, 2020

 

  1. Jitendra Singh Rathore “Vaccines” (2025) for M.Sc. Zoology (Core) Indra Gandhi National Open University, New Delhi 

 

  1. Jitendra Singh rathore “Antibody Engineering” (2025) for M.Sc. Zoology (Core) Indra Gandhi National Open University, New Delhi.

 

Publication

MODEL SUBMITTED IN MODEL ARCHIVE


 

 

  1. http://www.modelarchive.org/doi/10.5452/ma-azcde (2016)
  2. http://www.modelarchive.org/doi/10.5452/ma-abgt9 (2016)

 

SEQUENCES SUBMITTED IN NCBI DATABASE:


 

1. Singh, J. and Banerjee, N. AAZ06276.( 2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative colicin like protein [Xenorhabdus nematophila] gi|70671870|gb|AAZ06276.1|[70671870]

 

2. Singh, J., and Banerjee, N. AAZ06277. (2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative hemagglutinin/hemolysin [Xenorhabdus nematophila gi|120433596|gb|AAZ06 277.1|[120433596] 


3. Singh, J., and Banerjee, N. AAZ06278. (2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative cytoplasmic protein [Xenorhabdus nematophila] gi|70671872|gb|AAZ06278.1|[70671872] 


4. Singh, J., Shau, A. K., and Banerjee, N. ABC24675. (2005) Nucleotide sequence submitted in the NCBI database. Direct submission. Glycerophosphoryldiesterphosphodiesteras precursor [Xenorhabdus nematophila]

 

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